Journal: Cells
Article Title: Characterization of Epithelial–Mesenchymal and Neuroendocrine Differentiation States in Pancreatic and Small Cell Ovarian Tumor Cells and Their Modulation by TGF-β1 and BMP-7
doi: 10.3390/cells13232010
Figure Lengend Snippet: Relative expression of NED markers in PDAC-derived cell lines, PANC-1, MIA PaCa-2, and BxPC-3, and in the normal pancreatic duct epithelial cell line, HPDE. The panNET-derived cell lines, BON and NT-3, were employed here as controls. Cells were lysed at different times during continuous culture and subjected to RNA isolation and analyzed by qPCR. Expression levels for the indicated genes are displayed relative to those in PANC-1 cells, set arbitrarily at 1.0. Data represent the means ± SD of 3–6 independent preparations. Please note the extra scales for BON and NT-3 cells in the SSTR2, 5 and CHGA graphs, which indicate the orders of magnitude higher expression. Data shown are the mean ± SD of at least three independent experiments. The asterisks (∗) denote significant differences (two-tailed unpaired Student’s t -test). The asterisks above BON and NT-3 cells indicate a significant difference relative to PANC-1 cells.
Article Snippet: HPDE6c7 (HPDE) were purchased from AddexBio (#T0018001).
Techniques: Expressing, Derivative Assay, Isolation, Two Tailed Test